1. I have come to an incredibly liberating conclusion. About 50% of all experiments are not going to work. Maybe the technique is flawed, maybe there is something wrong with the reagents, maybe there is human error; for whatever reason, it just isn't going to work. This is nothing to get depressed about. There are plenty more experiments to do. Most of the time these dead experiments don't even deserve trouble-shooting: just drop it and move on; try something else. Hey, there's a 50% chance it'll work!
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